ID: 7055948 (Ref.No. 168732SM)
Posted: February 14, 2019
Application Deadline: Open Until Filled
Participate in a project to study the function of maternal effect genes that play a role in fertility, reproductive success and trophoblast differentiation. We are specifically interested in genes that encode proteins of the subcortical maternal complex of the oocyte and early embryo. The project primarily uses cell culture, including mouse and human embryonic stem cells, and mouse models. The project will involve investigating how they affect early development and reproductive outcomes via epigenetic mechanisms. The postdoc will work on NIH-funded projects to characterize the consequences of inactivation these proteins in embryonic stem cells and mice. Experiments will involve advanced cell culture methods, high throughput epigenomics and expression profiling and appropriate validation studies. Mouse breeding, including in vitro fertilization and, embryo transfer and cross fostering of offspring, detailed placenta and embryo characterization.
The impact of this research will be to better understand pathways and molecules involved in early embryo and trophoblast development and how maternal mutations contribute to abnormal reproductive outcomes, which may ultimately lead to better diagnosis and treatment of these conditions.
The postdoctoral research position is in Dr. Van den Veyver’s research laboratory in the Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital and Baylor College of Medicine
- PhD, MD/PhD or MD degree and a strong background in reproductive genetics.
- Experience with embryonic stem cell culture and differentiation protocols.
- Ability to work independently and take a leading role on the assigned project, under supervision and guidance of the P.I.
- Able to participate in supervision of less experienced lab members.
The following skills are highly desired for this position:
- Experience with DNA & RNA Extraction and analysis, cell culture, cloning, transfection, PCR, including qRT-PCR, tissue dissection and preparation for histology, in situ hybridization, immunostaining, protein analysis by western blotting and immunoprecipitation, mouse colony management and breeding.
- Experience with thorough experimental design, including sample size assessment, statistical analysis and interpretation, scientific database skills.
- Experience with experimental design for high-throughput sequencing-based expression and epigenomic profiling and their data analysis, which will be done with assistance of core facilities and bioinformatics collaborators.
Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.