ID: 7057873 (Ref.No. 304836DP)
Posted: June 14, 2019
Application Deadline: Open Until Filled
We are seeking a highly motivated individual for a post-doctoral position within the Laboratory for Regenerative Tissue Repair at the Baylor College of Medicine. The laboratory is focused on furthering our understanding of the molecular mechanisms that underlie the fetus’ ability to regeneratively heal cutaneous wounds and the development of novel therapies to achieve scarless wound healing in postnatal tissues. The laboratories within the center perform basic, translational, and pre-clinical research on the crosstalk between extracellular microenvironment, immunoregulation and mechanotransduction in tissue repair outcomes. The position currently available is to study the biogenesis, composition, structure and function of exosomes and microparticles in the skin and scars using tissue culture, and small animal model systems of wound healing. We will further explore the role of exosomes as delivery vehicles for targeted gene regulation. You will have the support of an academic advisor and scientific advisory committee who will provide you with the guidance and knowledge you need to develop your career. This is an exciting area that is not studied to its full potential, making this an opportunity to make a real difference to the future of regenerative medicine.
- Master's degree in a basic science or equivalent*.
- At least 2 years of prior lab experience and familiarity with molecular and cellular biology techniques.
*NOTE: A Bachelor's degree in a basic science and two years of directly related experience is considered 'equivalent' to a Master's degree.
- Recent PhD.
- An understanding of biochemistry, genetics and cell and molecular biology.
- Genetic perturbation techniques: CRISPR/Cas9, shRNA, etc.
- Prior work with small animal model systems.
- Practical experience of fluorescence/luminescence-based cell reporter assays.
- Mammalian cell culture, DNA, RNA and protein extraction and associated molecular techniques such as (RT)PCR, Western blotting, etc...
- In vitro cell culture assays such as cell proliferation, migration/invasion, apoptosis/cell cycle and differentiation
- Flow cytometry and FACS studies
- Cell imaging, confocal microscopy
- Immunofluorescence and IHC staining
- Standard molecular biology techniques, gene manipulation, Chip, cloning of plasmids, lentiviral vector production and complete characterization of transduction and cloning efficacy
- Experience of isolating, characterizing exosomes from cell/ tissue cultures,
- Experience in small animal surgery.
- Excellent influencing and problem solving skills, as well as the confidence to work independently with interdisciplinary group of scientists, physicians and engineers
- A proven record of publications in peer-reviewed journals and presentations at scientific conferences.