Research Assistant II Lab

Harvard University

Boston, MA

ID: 7062624
Posted: December 6, 2019
Application Deadline: Open Until Filled

Job Description

Job Summary

The Department of Immunology and Infectious Diseases (IID) focuses on the biological, immunological, epidemiological and ecological aspects of viral, bacterial and protozoan diseases of animals and humans, including the vectors that transmit infectious agents. Research in the department's 14 laboratories is primarily focused on diseases of developing countries.

The Neafsey Lab ( uses genomic and transcriptomic sequence data to study vector-borne infectious diseases (principally malaria). The lab employs a perspective informed by population genetics and molecular evolution to investigate interactions between microbes, vectors, and hosts.

Job-Specific Responsibilities

The successful candidate will assist in research exploring the evolutionary genomics of malaria parasites and Anopheles mosquito vectors.

The responsibilities of the Research Assistant include, but are not limited to, the following:
Performing molecular protocols preparatory to sequencing or genotyping samples, including DNA extraction, PCR or whole genome amplification, library construction, and DNA quantitation.
Execution and optimization of genotyping assays for malaria parasites and Anopheles mosquitoes, including locus selection, primer design, and evaluation of assay performance from Illumina sequencing data
Supporting tissue culture of Plasmodium falciparum, including preparation of media and culture reagents, long-term storage and retrieval of cell lines, culture adaptation of patient derived samples and subcloning, drug testing and other phenotypic assays, and isolation of nucleic acids (DNA and RNA) and protein samples.
Executing basic molecular biology techniques including DNA extraction, gene cloning, transfection, and analysis of DNA using standard laboratory techniques.
Managing samples and data with precision, and perform routine computational activities to summarize biological data
Other duties as assigned
The candidate is required to interact with a large variety of other staff and researchers including graduate students, postdoctoral fellows and senior scientists. The candidate will present his/her work in group and laboratory meetings to update the Principal Investigator.
PLEASE NOTE: This position has a term appointment of December 31, 2020, with the possibility of extension.

Basic Qualifications

2+ years of related experience required; combination of education and experience may be considered

Additional Qualifications

Bachelor's Degree preferred
College coursework in Biology or related field strongly preferred
Previous experience with vector-borne diseases a strong plus
Experience with sterile technique and basic molecular biology protocols preferred
Experience with or knowledge of molecular biology methods preferred
Tissue culture expertise is desirable
Strong analytical and quantitative skills preferred
High level of initiative and works with little supervision preferred
Excellent organizational skills preferred
Strong attention to detail preferred
Strong communication skills, both written and verbal, preferred
Experience with multi-tasking in a fast-paced environment preferred
Excellent interpersonal skills and great at working in a team environment preferred

Additional Information

Harvard University requires pre-employment reference checks and background screenings.
This position has a 90 day orientation and review period.
The Harvard T.H. Chan School of Public Health does not provide visa sponsorship for administrative and staff positions.


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