Research Assistant III - Regenerative Cellular Engineering

Harvard Medical School

Boston, MA

ID: 7085834
Posted: September 21, 2021
Application Deadline: Open Until Filled

Job Description

Job Summary

About us:

The Wyss Institute’s mission is to transform healthcare and the environment by emulating the way nature builds. We harness the freedom of academia and the focus of industry in order to translate revolutionary technologies into commercial products and solutions. We support research that universities, companies, and venture capital firms don’t fund because they view it as too risky. We prefer to use the word “challenging,” and we love challenges. For more information, visit: http://wyss.harvard.edu/



About you:

You are a passionate, impact-motivated scientist and problem solver who relishes the opportunity to participate in fast-paced, dynamic research. You are focused on delivering impact through translational science and hope to one day join or found an early-stage biotech startup. You enjoy working with diverse groups of people and leveraging your expertise to create innovations that can change the world.



About the role:

We are looking for a research assistant to join our rapidly growing team within the Church lab at the Wyss Institute focused on engineering complex cellular therapies to address unmet medical needs across a variety of spaces. We have developed a pipeline leveraging DNA barcoding & next-generation-sequencing to perform in-vivo pooled discovery for complex cellular behaviors such as migration & trafficking. The Church lab has a history of effectively translating novel technology concepts into high-powered biotech startups focused on increasing innovation & productivity within the industry & addressing unmet needs for patients living with debilitating diseases. Reporting to the project leads, you will be responsible for the execution of key research studies involving in-vivo pooled functional genomics of iPSC-based satellite muscle cell therapies for applications in regenerative medicine.


Job-Specific Responsibilities

What you’ll do:

Maintain long-term mammalian cell cultures of immortalized cell lines, perform transfections, generate, titer, and annotate lentiviral/retroviral stocks
Obtain, culture, and differentiate iPSCs into satellite muscle cells. In collaboration with other lab members, develop and optimize in-house protocols for transduction of satellite muscle cells with retroviral/lentiviral stocks, as well as protocols for freezing/thawing/shipping of modified satellite muscle cells
Conduct preparative level transductions of satellite muscle cells with viral stocks and culture until point of murine injection
Aid in murine work including animal sacrifice, dissection, & large scale gDNA isolation from murine organs
Execute on library preparation, sequencing, and analysis workflows in collaboration with project leads
Perform standard sub-cloning activities as needed to modify viral vectors or include additional genes for study
Order supplies and perform support activities and lab maintenance
Accurately and thoroughly document experimental observations and protocols and help maintain a high level of organizational efficiency within the lab
Demonstrate a commitment to diversity, inclusion, and cultural awareness in the workplace through actions & communications with others

Basic Qualifications

What you’ll need:

Bachelor’s degree in Biology, Bioengineering, Biochemistry, or related field is required.
At least 2 years of experience performing long-term mammalian cell culture and standard molecular biology techniques is required. Advanced education may count towards years of experience.

Additional Qualifications and Skills

Desirable skills:

Long-term mammalian cell culture of immortalized and primary cell lines is strongly preferred
Prior experience with human iPSC isolation, differentiation, and culture is advantageous, but not required
Familiarity with standard cloning techniques including gateway, gibson, and golden gate assembly is strongly preferred
Experience with mammalian cell transfection & lentiviral or retroviral production is preferred
Flow cytometry is strongly preferred
Familiarity with library preparation, NGS workflows & data analysis is preferred
Familiarity with python, R or similar programming language is preferred
Highly effective organizational and communication skills are essential

Harvard Medical School strives to cultivate an environment that promotes inclusiveness and collaboration among students, faculty and staff and to create new avenues for discussion that will advance our shared mission to improve the health of people throughout the world.



 

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