Postdoctoral Research Fellow, Cancer Immunology Discovery
La Jolla, CA
ID: 7104459 (Ref.No. 4846870)
Posted: July 1, 2022
Application Deadline: Open Until Filled
Job DescriptionWhy Patients Need You
Pfizer’s purpose is to deliver breakthroughs that change patients’ lives. Research and Development is at the heart of fulfilling Pfizer’s purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.
The Cancer Immunology Discovery group at Pfizer is seeking an ambitious Post-Doctoral Research Scientist with strong research and communication skills to undertake a highly visible research project shared between the Tumor Immunogenicity and T cell activation and PDx resistance groups. The successful candidate will study the impact of cytokines on CD8 T cell exhaustion in the tumor microenvironment. The scope of this work will potentially include the use of unique in vitro T cell systems and the use of a novel transgenic mouse reporter/lineage trace model that has yet to be characterized or published.
We are seeking a self-starting, highly motivated, and technically proficient cellular immunologist with a PhD to apply a variety of cutting-edge tools including molecular, cellular, and biochemical techniques alongside immunofluorescence imaging, high parameter flow cytometry, and bioinformatics in preclinical in vivo models to address key questions within the scope of the proposed work. (S)he will work in a fast-paced and highly collaborative research environment and will be expected to present their findings in high-impact journals and at internal and external scientific conferences.
PhD degree with experience in Immunology, Oncology, or related discipline.
A high-level understanding of key concepts in cancer immunology and T cell biology
A strong desire to learn, it is not expected that the successful candidate will have experience with all of the required techniques; however, they must be willing to expand their technical and scientific capabilities
Expertise in multiparameter flow cytometry, ex vivo mouse tissue processing, and primary mouse immune cell culturing
Experience in mouse handling and primary cell tissue harvest
Expertise in mouse and human T cell purification
Significant experience with standard T cell activation assays (e.g. peptide, anti-CD3, PMA/ionomycin) and readouts like intracellular cytokine FACS-staining, proliferation, ELISA, ELISPOT, and/or cytotoxicity.
Scientific and technical knowledge of T cell immunology, specifically the field of T cell dysfunction/exhaustion
Significant molecular biology experience including CRISPR-mediated deletion of genes in primary mouse cells
Expertise in bulk and single cell RNA-Seq analyses
Standing, sitting, bending, lifting, walking in a laboratory or vivarium setting.
Use of Personal Protective Equipment (PPE) in a BSL-2 laboratory; additional PPE requirements when working in vivarium barrier suites.
Standard business office conditions; sitting, standing as appropriate
OTHER JOB DETAILS
Eligible for Relocation Assistance
Must have Work Authorization in the US
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.