The Department of Cell Biology has an outstanding opportunity for a Laboratory Technician focused on cell biology and human genetics. This position is perfect for you if you’re interested in an entry-level laboratory job with the possibility for advancement.

About the Van De Weghe Lab: The laboratory conducts NIH-funded research focused on understanding how the cellular organelles, cilia, work normally and what goes wrong in disease. We employ both human cell systems and the green algae, Chlamydomonas, to probe function of ciliary proteins. We use microscopy, genome engineering, biochemical isolation of cilia, functional assays, and molecular biology approaches. We’re a new group committed to fostering diversity in the fields of cellular biology and genetics. We’re looking for an enthusiastic addition to our growing group. Come join us as we tackle exciting questions in Cell Biology.

Duties:

• Performs routine tests and analyses in a laboratory using appropriate technical equipment and instruments.
• Performs diagnostic laboratory tests and examinations according to standardized procedures, records and reports results.
• Performs chemical, physical, or biological tests and supports scientific investigators in research projects.
• Operates laboratory equipment, to include but not limited to centrifuges, titrators, pipetting machines and pH meters.
• Plans, sets up, and undertakes controlled experiments and trials.
• Records and analyzes data.
• Calculates test results, logs, and reports results to the supervisor.
• Compiles, enters, and graphs data and files reports. Inspects and makes minor repairs to laboratory equipment, assesses lab needs to order and stocks supplies and orders equipment.
• Media; making media and growth plates for Chlamydomonas cultures.
• Aliquots; making aliquots of reagents & ensuring stock.
• Stocking; keeping working spaces stocked with supplies.
• Upkeep; weekly/monthly checks and maintenance of equipment.
• Cleaning & autoclaving; various lab supplies as needed.
• Lab safety; support strict lab safety practices as related to wet chemicals, compressed gases, tissue culture, and hazardous materials, and support the laboratory's compliance with federal and state safety regulations.
• Ordering; ensure that all supplies for the lab are stocked accordingly.
• Lab organization; help keep the laboratory tidy and organized.
• Performs other duties as assigned to successfully fulfill the function of the position.

Possibility to learn:

• Tissue culture: Maintain human cell lines using sterile techniques.
• Chlamydomonas culture: Maintain algae using sterile techniques.
• CRISPR/Cas9 genome engineering: Use genome engineering techniques to model disease variants and introduce fluorescent proteins.
• Microscopy: analyze cell lines for defects using immunofluorescence, live cell tracking.
• Basic molecular biology techniques: nucleic acid isolation, PCR, qPCR, western blotting and other established techniques.
• Expertise: Stay up to date on research related to their projects by reading the literature and attending selected seminars.
• Data Presentation: summarize research results in presentations for lab meetings as well as for use in public presentations and publications.
• Interprets and analyzes data and discusses with principal investigator.
• Maintain appropriate research documentation and databases.