Research Technician II - Car T cells

Baylor College of Medicine

Houston, TX

ID: 7248101 (Ref.No. 19497-en_US)
Posted: May 26, 2024

Job Description


The lab is looking for highly motivated candidates to join a team of scientists, physicians, postdoctoral fellows, technicians, and graduate students to support research on cellular immunotherapy at the department of Pediatrics, Hematology-Oncology cell and gene therapy at BCM. The technician will provide responsible technical assistance in support of research projects by completing assignments requiring a basic knowledge of fundamental techniques and practices related to basic and translational research in cellular immunotherapy of cancer. The technician will work under supervision in accordance with general plans approved by the principal investigator. Salaries are commensurate with a candidate's experience level.

Job Duties

  • Optimizes and performs research experiments to produce reliable and precise data to support scientific investigations.
  • Records, summarizes and analyzes research data generated from experiments.
  • Conducts aseptic cell culture for production and propagation of tumor lines, CAR T cells and antigen-specific T cells.
  • Performs PCR, cloning and retroviral/lentiviral transduction for genetic modification of T cells and tumor lines.
  • Performs flow cytometry and immunological assays including multiplex assays, ELISA, ELISPOT and Western blotting.
  • Works with small rodent models of cancer immunotherapy.
  • Works on immunohistochemistry, immunocytochemistry and image analysis using scientific imaging software.
  • Uses statistical packages like R or Graph-pad Prism to plot and analyze experiential data.
  • Uses office suite, instrument interface and other software for data collation, compilation, and analysis.
  • Performs research experiments to produce reliable and precise data to support scientific investigations in cell and gene therapy applications under the supervision of principal investigator.
  • Prepares figures and analyzes for presentations and publications.
  • Coordinates ordering, shipping, and receiving of research materials.
  • Coordinates with other users on instrument and core facility use.
  • Performs other job-related duties as assigned.

Minimum Qualifications

  • Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
  • No experience required.

Preferred Qualifications

  • Biochemistry/Cell biology/Biotechnology/Immunology/Any life Sciences.
  • Experience full time/part time working in research laboratory.
  • Experience working in aseptic cell culture techniques, Retroviral/lentiviral transduction, flow cytometry, RT-PCR, IHC, ICC, small rodent handling, bioinformatics background and working knowledge of statistical packages like R or Prism.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

Baylor College of Medicine fosters diversity among its students, trainees, faculty and staff as a prerequisite to accomplishing our institutional mission, and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care. - Diversity, respect, and inclusiveness create an environment that is conducive to academic excellence, and strengthens our institution by increasing talent, encouraging creativity, and ensuring a broader perspective. - Diversity helps position Baylor to reduce disparities in health and healthcare access and to better address the needs of the community we serve. - Baylor is committed to recruiting and retaining outstanding students, trainees, faculty and staff from diverse backgrounds by providing a welcoming, supportive learning environment for all members of the Baylor community.